Identification of deubiquitinase inhibitors via high-throughput screening using a fluorogenic ubiquitin-rhodamine assay
Summary: Identification of selective deubiquitinase (DUB) inhibitors is critical for probe development to further cashel tail bag understand and explore DUB biological function.Here, we detail the optimization and deployment of an in vitro fluorogenic ubiquitin-rhodamine assay to conduct high-throughput screening of a small molecule library against